Histology of the Respiratory System
Examine the following slides and illustrate the views, labeling the specified features. The page numbers (VE) are in di Fiore’s Atlas of Normal Histology, 9th Ed:
Trachea, c.s., Slide 5 (H 2430), VE: p. 243, 40x
Layers:
1) (pink)pseudostratified ciliated columnar epithelium and lamina propria
2) (bluish-purple) tracheal rings, composed of hyaline cartilage
3) (pink) adjacent layers of perichondrium
4) (bluish) another layer of hyaline cartilage
5) (pink) adventitia: connective tissue surrounding the trachea
Trachea, c.s., Slide 5 (H 2430), VE: p. 243, 400x
pseudostratified ciliated columnar epithelium
cilia
goblet cell
basement membrane
lamina propria
tracheal glands: secrete mucus
mucous alveoli: large pale blue-gray cytoplasm, nuclei at periphery ducts of tracheal glands (lined with simple cuboid)
perichondrium
hyaline cartilage
chondrocytes
Here is a labeled view of tracheal histology.
bronchus: VE: p. 247, at 40x: This overview shows the bronchus embedded in a field of aveoli.
bronchus: find one with noted features, VE: p. 247, at 100x: alveoli
bronchus or bronchiole: significance or characteristics:
mucosal folds pseudostratified ciliated columnar epithelium
lamina propria connective tissue underlying mucous membrane
smooth muscle forms a smooth pink ring outside mucosa
adventitia often filled with lymphoid tissue
lymph node nuclei numerous, stained blue-purple
pulmonary artery thick walled
pulmonary vein thin walled, collapsed, if present
Here is a labeled view of a bronchus.
bronchus: VE: p. 247, at 400x:
mucosal folds are clear
pseudostratified ciliated columnar epithelium is more distinct
lamina propria connective tissue underlying mucous membrane
smooth muscle forms a smooth pink ring outside mucosa
Lung, l.s., Slide 4 (H 2460), page 245 & 247:
at edge of lung: at 400x
visceral pleura mesothelium covering the surface of the lung
alveoli
blood vessel (hard to spot, incorporated into alveolar wall, look for RBCs.)
respiratory bronchiole, if present (lined with cuboid epithelium)
Here are some images of lung pathologies (all about 100x):
Emphysema
Pneumonia
Tuberculosis
Addtional images with other magnifications of the specimens.
Lung, l.s., Slide 4 (H 2460), page 245 & 247:
at edge of lung: at 600x
Look carefully and you can see erythrocytes in the lower right of the alveolus.
Here is a labeled view of the edge of the lung.
