Introduction to Nervous Tissue Histology

Introduction to Nervous Tissue Histology

Examine the following slides and identify the listed features. Survey each slide for the best typical field which shows the listed features, and illustrate it at the indicated power, labeling these features. At home, fill in the significance of each feature in a sentence or two. Follow protocol Notebook Illustrations. (MF 9 th= Eroschenko’s 9th Ed)

Slide 1: Spinalmotor nervecell, ox, hematoxylin-eosin stain (H 1660)
(MF 9 th , page 89) at 400x:
scan with 4x objective, find a neuron which best shows the following, draw at 400x:

Neuron: the functional unit of nervous system
Identify the following features:
nucleus houses genetic material
nucleolus synthesizes ribosomal RNA, assembles ribosomes. Prominent in neurons.
Nissl bodies rough endoplasmic reticulum (synthesizes protein). Prominent in cytoplasm.
perikaryon also known as cell body, equals the area “around the nucleus”
axon hillock often tapered, gives rise to the axon, pale or clear due to lack of Nissl bodies
dendrites darker stained projection, carries impulses toward cell body. Can be multiple.
axon clearer projection, carries impulses away from cell body
astrocytes most common neuroglial cells, support, nourish neurons
microglia cells with large nuclei dispersed among astrocytes (scarce), phagocytic

Here is an older version of a labeled version of the 400x image of a neuron.

Slide 6: Motor nerve endings with end plates, snake, AuCl 2 stain (H 1685)
Motor end plates, known also as neuromuscular junctions
(MF 9 th , page 81) at 400x:

Identify the following features:
skeletal muscle also known as striated muscle, bands of sarcomeres visible
nerve bundle axons bound together by Schwann cells (not visible)
axon delivers impulses to the motor end plate
motor end plate a cluster of synaptic knobs attached to muscle fibers
synaptic knobs (or boutons) release acetylcholine, the neuromuscular neurotransmitter

Here is a labeled version of 400x view of a motor end plate.
Here is an older version of a labeled of 400x view of a motor end plate.

cut here ———————————————–


Slide 10: Vater-Pacini corpuscles, pancreas, H-eosin (H 1688)
Pacinian corpuscles, specialized nerve ending which detects pressure and vibration in an organ.
(MF 9 th: page 229 and 147) at 100x:
Pacinian corpuscle:
connective tissue capsule
central cavity with numerous lamellae (with flattened nuclei) and naked dendrite
Pancreatic acini


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