Histology of the Eye

Histology of the Eye

Follow protocol Notebook Illustrations

This is another slide in which, because of the size of the specimen, you will have to move around to see the entire structure, even at the lowest power. First look at the slide with the naked eye to orient yourself to its gross features: you should be able to see the cornea, lens (often fragmented on the interior), cavities, and ciliary bodies. Note that some slides are oriented R and L, others up and down…

With the 4x objective, find the sclera and follow it around the entire perimeter, identifying the regions as you come to them and noting the layers in each region. Again, identify all features listed before you begin an illustration. You will make three illustrations:

  1. The gross anatomy of the anterior portion
  2. A low power view of the rear wall
  3. A high power view of the retina:

Slide 8:  Eye, Monkey, general structure, sagittal section, general features H 1064  (MF 4th, page 343, 345)

Eyeball_PC271488
Section through the eye optic nerve on the left,25x

At 25x: Gross anatomy of section through the eye:    (Here is a labeled version of the eye section.)
FIBROUS TUNIC
cornea (anterior 1/6th)
sclera (posterior 5/6ths)
ocular conjunctiva
corneal limbus (cornea & sclera join)
canal of Schlemm (poss. not visible)

VASCULAR TUNIC:
ciliary body:
ciliary muscle
ciliary processes
iris
pupil

NERVOUS TUNIC:
retina
lens (It is often fragmented during slide preparation, and only the outline remains.)
suspensory ligaments (not visible?)
anterior cavity
anterior chamber
posterior chamber
aqueous humor
posterior cavity
vitreous humor

eyeball_anterior_half_PC271489

At 40x: Gross anatomy of anterior portion of the eye
Here is a labeled view of the anterior portion of the eye.

vascular_tunic_lens_PC271490

VASCULAR TUNIC, 60x:
ciliary body:
ciliary muscle
ciliary processes
iris
pupil

vascular_tunic_PC271519

VASCULAR TUNIC, 100x: (Here is a labeled version of the anterior vascular tunic and fibrous tunic.)
 ciliary body:
ciliary muscle
ciliary processes
iris
pupil

cornea_200x_PC271515

400x view of cornea, left to right:
posterior epithelium (low cuboidal cells)
Corneal stroma withkeratocytes embedded in collagen fibers
anterior epithelium (stratified squamous epithelium)

optic_nerve_PC271516

At 100x: cross section through rear wall of eye with optic nerve: Here is a labeled view of the rear wall.
 left to right:
optic nerve with parallel axons from ganglion cells
optic disc  (note photoreceptors are absent in retina where the optic nerve is attached)  adventitia (orbital fatty tissue)
sclera
choroid, with melanocytes
retina:
cell bodies of
photoreceptors
bipolar cells
ganglion cells
Here is an extraordinarily good image of the blind spot where the optic nerve exits the rear of the eye.

eye_rear_wallPC271514

300x:  view through the rear wall of the eye showing, top to bottom:
Nervous tunic
ganglion cell bodies
bipolar cell bodies
photoreceptor cell bodies
Vascular tunic:
pigmented cells of choroid
choroid
Fibrous tunic:
sclera

retina_PC271518
Section through the retina 400x

400x view of cross section of retina:     (Here is a labeled version of the cross section of the retina.)
Deep to superficial tissues (left to right):
pigment cells
rods (finer and longer)
cones (thicker and shorter)
outer limiting membrane
nuclei of cones (closer to choroid)
nuclei of rods (further from choroid)
nuclei of:
horizontal cells (closest to receptors)
bipolar cells
amacrine cells (closest to ganglion)
ganglion cell bodiesMullers fibers (vertical fr ganglion cells)

Other images related to the eye:

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