Epithelial Tissues Lab

Epithelial Tissues Lab

First review and follow carefully the rules and regulations for microscope use. Also, be familiar with guidelines in Notebook Illustrations for these drawings.
Examine each of the following slides, note the features in common and those which are differentiating. Illustrate each at 400x to take up most of a page. Include and label each listed feature, and give a brief description of its function or significance. Compare with the plates in Eroschenko’s Atlas of Normal Human Histology, 9th Ed (VE).

Simple squamous epithelium
Simple squamous epithelium

Slide 2: Simple squamous epithelium, (VE:11): top view of peritoneum, a serous membrane (an example of mesothelium, derived from mesoderm). It functions where a slick friction-free surface is required, or ready exchange across membranes is essential: lung alveoli, capillary endothelium, kidney glomerulus.

Features to label in your illustration:
cell borders
nucleus
nucleolus
tiled appearance (its heterogeneity is due to variations in sample preparation)

Here is a labeled view of simple squamous epithelium.


Simple cuboid epithelium
Simple cuboid epithelium

Slide 3 Simple cuboid epithelium, (VE:257) section of kidney. In the cortex (outer portion) of the section, view the proximal convoluted tubules with brush borders.

Features to label in your illustration:
proximal convoluted tubule
basement membrane
nuclei
lumen
brush border (only on proximal convoluted tubules)

Here is a labeled view of simple cuboid epithelium.


Simple columnar epithelium
Simple columnar epithelium

Slide 4 Simple columnar epithelium, (VE:13) c.s. of the intestine of a Nectarus (a newt).
Note the classic four functional layers of a GI wall, inside to outside: mucosa, submucosa, muscularis, and serosa.

Here is a 40x view of the villi.

Features to label in your illustration:
basement membrane
brush border
nuclei
goblet cells
lamina propria (connective tissue which underlies mucous membrane)
capillaries in lamina propria (lacking in epithelium)
Here is a labeled view of simple columnar epithelium.


Stratified squamous epithelium
Stratified squamous epithelium

Slide 5 Stratified squamous epithelium,(VE:17) dog esophagus. This tissue is especially resistant to friction. Note the mucous alveoli of the esophageal glands.

Features to label in your illustration:
basement membrane
basal cells (brownish cells along basement membrane where mitosis occurs)
squamous cells being shed (superficially, include nucleated surface cells)
lamina propria

Here is a labeled view of stratified squamous epithelium.


Pseudostratified ciliated columnar epithelium
Pseudostratified ciliated columnar epithelium

Slide 6 Pseudostratified ciliated columnar epithelium, c.s. of trachea , (try slide 11 if the detail is poor) (VE:15) This tissue is especially prominent in the respiratory tree.

Features to label in your illustration:
basement membrane
numerous nuclei in deeper half of the tissue
goblet cells
cilia (contrast with brush border for appearance & function)
lamina propria

Here is a labeled view of pseudostratified columnar epithelium.

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