Here are pdfs for lecture notes and lab protocols (please do not reproduce without written permission):
LECTURE NOTES: Here is a directory of informal lecture notes for my course. Please read the paragraph below.**
LABORATORY PROTOCOLS AND HANDOUTS: a directory of lab handouts.
BELOW ARE ILLUSTRATED PAGES FOR THE HANDOUTS CREATED FOR THIS COURSE.
PLEASE DO NOT REPRODUCE WITHOUT WRITTEN PERMISSION.
The following protocols have been written by David Fankhauser for his Microbiology Course at the University of Cincinnati Clermont College.
They should not be reproduced without his express written permission. Thank you.
1) Table of Contents
2) Micro 2031 2015 Fall Semester Course Syllabus
3) How to Take a Fankhauser Course
4) Study Groups: Towards Effective Peer Education
5) Study Group Report Form
6) Micro Wordstems I, first half of quarter
7) Micro Wordstems II, second half of quarter
8) Micro Cumulative Wordstems List with meanings
FOR THE LAB NOTEBOOK:
9) Lab Schedule for Microbiology 2031, Spring 2015
10) Laboratory Notebook Procedure (Here is a pdf of the handout, 2012) Here is an illustrated page devoted to setting up your Microbiology Lab book.
11) Format for Table of Contents
12) Use of Contact Paper for Mounting Handouts
13) Sample First Notebook Gradesheet from previous year
14) Notebook Illustrations
15) Making Root Beer at Home
15b) Bacterial Colonies on Potato Slices a la Koch
16) Index to Micro Slides
17a) Binocular Microscope: Its Features and Care
17b) Microscope Storage Gradesheet
18a) How to View a Slide
18b) Bacterial Morphology for Microbiology Students
19) Sample Math Problems for Microbiology
20) Use of Oil Immersion Objective
21) Equipment for a Microbiology Workstation
22) Bacteriological Smear and Staining Protocol
23) Buccal Smear
24) Bacterial Flora of Teeth
25) Microbiological Media Preparation
26) Commonly Used Solid Media and Other Common Microbiological Media
27) Other Commonly Used Media for Phage Growth
28) Autoclave Use
29) Bacterial Growth Curve: E coli on various types of liquid media
30) Spectrophotometer Use
31) Graph Construction (Here is a pdf of an example notebook page demonstrating Graph Construction.)
32 & 33) Two sheets of three cycle semi-log paper
34) Preparation of Wet Mount Slide
35) Gram Stain Protocol
36) Milk Fermenters
37) Dilutions with Sample Problems
38) Serial Dilution, Pipetting Practice
39) Displacement Pipetters: Their Care and Use
40) Sterile Technique: Delivery of Liquids by Pipette
41) Spreading Technique for Plating Bacteria
42) Yeast Plate Count Protocol
43) Bacterial Anatomy (on Prepared Slides)
45) Table of Contents, Part 2
46) Lab Schedule for Microbiology 2031, Second half
47) Microbiology Wordstems, Second Half of Quarter
48) Sample Second Note Book Grade Sheet from previous year
49) Agar Overlay Technique
50) Demonstration of Bacterial Growth Inhibition
51) UV Killing of Bacteria
52) Additional Experiments with the UV Killing Assay
53) Hemolytic Streptococcus Detection by Throat Culture
54) Assay for Coliform Contamination in Ambient Water
55) Single Colony Isolation
56) Triple Sugar Iron Agar and Its Use
57) Triple Sugar Iron Agar: Interpretation of Results
58) Pour Plate Technique for Bacterial Enumeration
59) Bacterial Contamination of Milk: Pour Plate Assay
60) Bacterial Contamination of Meat: Pour Plate Assay
61) Meat and Milk Assay Setup
62) Protozoan and Helminth Parasites
63) Protozoan Phyla and Examples (not required for NB)
64) Salmonella Detection
Preparation of Media for Salmonella Detection
66) Coliform in Drinking Water Assay, p 1
67) Coliform in Drinking Water Assay, p 2
68) Preparation of Bacteriophage Stocks
69) Titering of Bacterial Viruses
LINKS: Questions about microbes? Here is a rich resource page of information about microbes at microbes.info
**Here is a directory containing some of my lecture notes in *.pdf form. Note that they are outline form, and informal. If you see errors in them please let me know. Even with these notes printed out, I urge students to take their own detailed notes in class for several reasons. First, the act of writing is very important in creating a long term memory in your brain. Second, my lecture notes are only guidelines for my lectures. My students soon recognize that I often go on a tangent which may not be in my prepared notes. Third, when you review your own handwritten notes, you will see areas which are not clear, and provide you an opportunity to clarify the question at the beginning of the next class.
And here is the directory of *pdf files of my Microbiology Lab protocols.
The following are pages which contain links to web images related to lectures presented.
The History of Microbiology
The History of the Germ Theory of Disease.
Essentials of microscopy and staining.
Enzymology and Metabolism
Microbial Fermentation (Part of previous page…)
Requirements for Microbial Growth and Media
Control of Microbial Growth, Physical Means
Control of Microbial Growth, Chemical Means
Staphylococci and Streptococci
Spore Forming Bacteria
Gram Negative Rods and Cocci
Mycoplasmas, Rickettsias, Chlamydias, Spirochetes, Vibrios
Mammalian Pathogenic Viruses
Here is a page of illustrations related to the history of microbiology.