First Year Biology Lab 1082L
Table of Contents
I have not taught BioLab 1082L course in several years. Here are instructions for how to print out, cut and bind your First Year BioLab manual. Please print out the packet, cut it into 5.5 x 8.5 inch pages, and have it bound with spiral binding at a local copy store (Kinko’s, Office Max, etc. Costs about $4.50). I will give specific instructions on the first day of lab.
I will be adding and/or modifying many of these protocols as we work our way through the quarter. Stay tuned.
The following links will take you to illustrated pages to explain each topic in more detail and with images:
Handout: Students must mount these protocols in their notebooks. All students are responsible for them and should know them.
Table of Contents
Schedule of Lab Activities, Biology Lab 1082L, Spring Semester 2014
Binocular Care and Use
Writing a Scientific Paper
Making Labneh (simple yogurt Cheese)
Fungi Sporulation (Images Only, No Alt-Tag/Captions)
Mosses and Ferns (Images Only, No Alt-Tag/Captions)
Here are pdfs for lecture notes and lab protocols (please do not reproduce without written permission):
LECTURE NOTES: Here is a directory of informal lecture notes for my course. Please read the paragraph below.**
LABORATORY PROTOCOLS AND HANDOUTS: a directory of lab handouts.
BELOW ARE ILLUSTRATED PAGES FOR THE HANDOUTS CREATED FOR THIS COURSE.
PLEASE DO NOT REPRODUCE WITHOUT WRITTEN PERMISSION.
The following protocols have been written by David Fankhauser for his Microbiology Course at the University of Cincinnati Clermont College.
They should not be reproduced without his express written permission. Thank you.
1) Table of Contents
2) Micro 2031 2015 Fall Semester Course Syllabus
3) How to Take a Fankhauser Course
4) Study Groups: Towards Effective Peer Education
5) Study Group Report Form
6) Micro Wordstems I, first half of quarter
7) Micro Wordstems II, second half of quarter
8) Micro Cumulative Wordstems List with meanings
FOR THE LAB NOTEBOOK:
9) Lab Schedule for Microbiology 2031, Spring 2015
10) Laboratory Notebook Procedure (Here is a pdf of the handout, 2012) Here is an illustrated page devoted to setting up your Microbiology Lab book.
11) Format for Table of Contents
12) Use of Contact Paper for Mounting Handouts
13) Sample First Notebook Gradesheet from previous year
14) Notebook Illustrations
15) Making Root Beer at Home
15b) Bacterial Colonies on Potato Slices a la Koch
16) Index to Micro Slides
17a) Binocular Microscope: Its Features and Care
17b) Microscope Storage Gradesheet
18a) How to View a Slide
18b) Bacterial Morphology for Microbiology Students
19) Sample Math Problems for Microbiology
20) Use of Oil Immersion Objective
21) Equipment for a Microbiology Workstation
22) Bacteriological Smear and Staining Protocol
23) Buccal Smear
24) Bacterial Flora of Teeth
25) Microbiological Media Preparation
26) Commonly Used Solid Media and Other Common Microbiological Media
27) Other Commonly Used Media for Phage Growth
28) Autoclave Use
29) Bacterial Growth Curve: E coli on various types of liquid media
30) Spectrophotometer Use
31) Graph Construction (Here is a pdf of an example notebook page demonstrating Graph Construction.)
32 & 33) Two sheets of three cycle semi-log paper
34) Preparation of Wet Mount Slide
35) Gram Stain Protocol
36) Milk Fermenters
37) Dilutions with Sample Problems
38) Serial Dilution, Pipetting Practice
39) Displacement Pipetters: Their Care and Use
40) Sterile Technique: Delivery of Liquids by Pipette
41) Spreading Technique for Plating Bacteria
42) Yeast Plate Count Protocol
43) Bacterial Anatomy (on Prepared Slides)
45) Table of Contents, Part 2
46) Lab Schedule for Microbiology 2031, Second half
47) Microbiology Wordstems, Second Half of Quarter
48) Sample Second Note Book Grade Sheet from previous year
49) Agar Overlay Technique
50) Demonstration of Bacterial Growth Inhibition
51) UV Killing of Bacteria
52) Additional Experiments with the UV Killing Assay
53) Hemolytic Streptococcus Detection by Throat Culture
54) Assay for Coliform Contamination in Ambient Water
55) Single Colony Isolation
56) Triple Sugar Iron Agar and Its Use
57) Triple Sugar Iron Agar: Interpretation of Results
58) Pour Plate Technique for Bacterial Enumeration
59) Bacterial Contamination of Milk: Pour Plate Assay
60) Bacterial Contamination of Meat: Pour Plate Assay
61) Meat and Milk Assay Setup
62) Protozoan and Helminth Parasites
63) Protozoan Phyla and Examples (not required for NB)
64) Salmonella Detection
Preparation of Media for Salmonella Detection
66) Coliform in Drinking Water Assay, p 1
67) Coliform in Drinking Water Assay, p 2
68) Preparation of Bacteriophage Stocks
69) Titering of Bacterial Viruses
LINKS: Questions about microbes? Here is a rich resource page of information about microbes at microbes.info
**Here is a directory containing some of my lecture notes in *.pdf form. Note that they are outline form, and informal. If you see errors in them please let me know. Even with these notes printed out, I urge students to take their own detailed notes in class for several reasons. First, the act of writing is very important in creating a long term memory in your brain. Second, my lecture notes are only guidelines for my lectures. My students soon recognize that I often go on a tangent which may not be in my prepared notes. Third, when you review your own handwritten notes, you will see areas which are not clear, and provide you an opportunity to clarify the question at the beginning of the next class.
And here is the directory of *pdf files of my Microbiology Lab protocols.
The following are pages which contain links to web images related to lectures presented.
The History of Microbiology
The History of the Germ Theory of Disease.
Essentials of microscopy and staining.
Enzymology and Metabolism
Microbial Fermentation (Part of previous page…)
Requirements for Microbial Growth and Media
Control of Microbial Growth, Physical Means
Control of Microbial Growth, Chemical Means
Staphylococci and Streptococci
Spore Forming Bacteria
Gram Negative Rods and Cocci
Mycoplasmas, Rickettsias, Chlamydias, Spirochetes, Vibrios
Mammalian Pathogenic Viruses
Here is a page of illustrations related to the history of microbiology.
Microbiology 2031 Lab Handouts Directory
Gallery of Micrographs of Prepared Slides of Microbes
How to View a Slide
You are responsible for the welfare of the microscope in your numbered seat. Take care of it please. You may also have received a set of slides numbered according to your seat. You are responsible for their welfare also. Keep them clean and in good order. Check to see that they are in proper order and good condition, then sign your name on the label on the slide box cover to take responsibility for them.
You should be familiar with all the functional parts of the microscope as spelled out in Microscope Features and Care. Fill out the Microscope Check-In form in your desk drawer as you are instructed, date and sign your name showing it is in good working order. If the condition is anything but excellent, notify instructor.
How to view a slide:
Prepare the microscope
Lower the stage all the way with the coarse focus.
Set the oculars for your interpupillary distance, set adjustable ocular on zero (to start with).
Select the 4x objective if it is not already in position by rotating the turret.
Prepare the lighting:
Placing the slide on the microscope:
Handle all slides by the edges only.
Open the slide retainer by pressing the jaw lever to the right. Place the slide into the “L” shaped holder (with the label to the left if it is a prepared slide). Release the jaw lever to clamp the slide into the holder.
Center the specimen: Using the mechanical stage, move the slide until the stained specimen is directly over the light of the condenser (the optic center of the stage). View alternately from side and front to get a best estimate of center.
Viewing from the side, raise the stage until until the stage stops or the objective almost touches the slide.
Looking through the oculars, lower the stage with the coarse focus until the specimen comes into focus. Use the fine focus to refine the If it never appears, re-adjust the position of the specimen nearer the optic center, and repeat the 4x focusing procedure.
Scan the specimen to find a field that is characteristic, well spread out and stained. Center the most desirable region.
Rotate the nose piece to select 10x objective. (What is the power of the view?) Using only the fine focus, make minor focus adjustments to sharpen the image. Center the image again. (If you lose the view of the specimen, go back to the 4x objective to find it again and center more carefully. Is the view clear and bright?
Rotate the nose piece to select the 40x objective. Make minor adjustments in focus with fine focus. Center the desirable region. Adjust lighting with the condenser positioning knob and the iris diaphragm. If need be, nudge the rheostat higher to have optimum lighting.
To correct for differences between your R and L eyes, look through the right ocular with only the R eye and focus to sharpness. Then look through the L ocular with only the L eye, and rotate the L ocular until the image is sharp. If your eyes are closely matched, the diopter scale on the L ocular should read close to zero. Write the number in your book. Adjust interpupillary distance, write this number in your book as well. Label both clearly. Using the mechanical stage, move the slide to the L. Note that the image moves in the opposite direction of the actual slide. Is the view bright and clear? If so, write excellent under both 4x objective and ocular columns. If the view is hazy, clean with lens paper.
How to return the microscope to storage:
1: Lower stage fully
2: Return the 4x objective to the viewing position.
3: Reduce rheostat to lowest setting, turn off power switch
4: Wrap cord snugly, no sharp bends, tuck in plug.
5: Replace dust cover, return to storage, arm towards you.
6: Report any problem with the instrument immediately.